This example utilises a small portion of a dataset available in the PRIDE database. I am using the files with designation F048911 however any others would work too.

This proteomics dataset includes results from a GeLC-MS/MS experiments where a novel quantification method was developed that could be used as a Western Blot alternative. Feel free to read their article here.

Title: 20160216_FlyEye_D3012_Heavy_23ul_DionexUPLC_120min_02.05181.05181.3.dta

ID: Scan 1:1

Precursor sequence: ATAWVIVHR | Start: 474 | End: 482

Precursor m/z: 354.8745 | Precursor charge: 3+

Found fragments: 19

Protein description: Transient receptor potential protein OS=Drosophila melanogaster GN=trp PE=1 SV=3

Protein accession: sp|P19334|TRP_DROME

Sequence:

MGSNTESDAE KALGSRLDYD LMMAEEYILS DVEKNFILSC ERGDLPGVKK ILEEYQGTDK FNINCTDPMN RSALISAIEN 80

ENFDLMVILL EHNIEVGDAL LHAISEEYVE AVEELLQWEE TNHKEGQPYS WEAVDRSKST FTVDITPLIL AAHRNNYEIL 160

KILLDRGATL PMPHDVKCGC DECVTSQMTD SLRHSQSRIN AYRALSASSL IALSSRDPVL TAFQLSWELK RLQAMESEFR 240

AEYTEMRQMV QDFGTSLLDH ARTSMELEVM LNFNHEPSHD IWCLGQRQTL ERLKLAIRYK QKTFVAHPNV QQLLAAIWYD 320

GLPGFRRKQA SQQLMDVVKL GCSFPIYSLK YILAPDSEGA KFMRKPFVKF ITHSCSYMFF LMLLGAASLR VVQITFELLA 400

FPWMLTMLED WRKHERGSLP GPIELAIITY IMALIFEELK SLYSDGLFEY IMDLWNIVDY ISNMFYVTWI LCRATAWVIV 480

HRDLWFRGID PYFPREHWHP FDPMLLSEGA FAAGMVFSYL KLVHIFSINP HLGPLQVSLG RMIIDIIKFF FIYTLVLFAF 560

GCGLNQLLWY YAELEKNKCY HLHPDVADFD DQEKACTIWR RFSNLFETSQ SLFWASFGLV DLVSFDLAGI KSFTRFWALL 640

MFGSYSVINI IVLLNMLIAM MSNSYQIISE RADTEWKFAR SQLWMSYFED GGTIPPPFNL CPNMKMLRKT LGRKRPSRTK 720

SFMRKSMERA QTLHDKVMKL LVRRYITAEQ RRRDDYGITE DDIIEVRQDI SSLRFELLEI FTNNNWDVPD IEKKSQGVAR 800

TTKGKVMERR ILKDFQIGFV ENLKQEMSES ESGRDIFSSL AKVIGRKKTQ KGDKDWNAIA RKNTFASDPI GSKRSSMQRH 880

SQRSLRRKII EQANEGLQMN QTQLIEFNPN LGDVTRATRV AYVKFMRKKM AADEVSLADD EGAPNGEGEK KPLDASGSKK 960

SITSGGTGGG ASMLAAAALR ASVKNVDEKS GADGKPGTMG KPTDDKKAGD DKDKQQPPKD SKPSAGGPKP GDQKPTPGAG 1040

APKPQAAGTI SKPGESQKKD APAPPTKPGD TKPAAPKPGE SAKPEAAAKK EESSKTEASK PAATNGAAKS AAPSAPSDAK 1120

PDSKLKPGAA GAPEATKATN GASKPDEKKS GPEEPKKAAG DSKPGDDAKD KDKKPGDDKD KKPGDDKDKK PADNNDKKPA 1200

DDKDKKPGDD KDKKPGDDKD KKPSDDKDKK PADDKDKKPA AAPLKPAIKV GQSSAAAGGE RGKSTVTGRM ISGWL      1280